New Enzyme Immunoassay for Salivary Cortisol
نویسندگان
چکیده
منابع مشابه
New enzyme immunoassay for salivary cortisol.
concentration of a sample that gave an absorbance value within the linear range of the assay and was expressed relative to the absorbance/g of BSA (corresponding to the gradient of the calibration curve): Interference ϭ absorbance/g of aminoglycoside absorbance/g of BSA ϫ 100% The aminoglycosides gave negligible interference in the CBB assay (Ͻ0.2% relative to BSA) and failed to generate tur...
متن کاملHomogeneous enzyme immunoassay for cortisol with a centrifugal analyzer.
We determine serum cortisol by a homogeneous enzyme immunoassay in the Cobas Bio centrifugal analyzer. To unbind cortisol from its protein complex, serum is treated for 15 min with an acidic solution. The reaction then proceeds automatically in the analyzer at 37 degrees C. To 50 microL of sample mixture is added 125 microL of reagent (cortisol antibodies, glucose 6-phosphate, and NAD+). This m...
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Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
متن کاملSalivary cortisol determined by enzyme immunoassay is preferable to serum total cortisol for assessment of dynamic hypothalamic--pituitary--adrenal axis activity.
OBJECTIVE The aim of this study was to determine whether salivary cortisol measured by a simple enzyme immunoassay (EIA) could be used as a surrogate for serum total cortisol in response to rapid changes and across a wide range of concentrations. DESIGN Comparisons of matched salivary and serum samples in response to dynamic hypothalamic-pituitary-adrenal (HPA) axis testing. Subjects Healthy ...
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A competitive, double antibody enzyme immunoassay for oxytocin in a heterologous system was developed. Horseradish peroxidase was conjugated with oxytocin using N-succinimidyl 3-(2-pyridyldithio) propionate, and rabbit anti-oxytocin serum was produced by immunization of oxytocin-bovine serum albumin complex which was prepared by the carbodiimide method. The sensitivity of the assay was 4 microI...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2003
ISSN: 0009-9147,1530-8561
DOI: 10.1373/49.1.203